Print Single Cell World tweet on a t-shirt
@singlecellworld
DROPLET-based methods are the most commonly used in #singlecell research
🤔 BUT are the best? Not always.
Studying mRNA isoforms? 👉🏼PLATE-based methods
Studying neutrophils/ granulocytes? 👉🏼 (micro- nano- or pico- well based methods)
Other Single Cell World tweets to print
@singlecellworld
Terminology of the week 👉🏻 Bulk Experiment
🟢 Standard experiment where we analyse a bunch of cells that are together in the same tube.
🟢The result is the average of what is going on in ALL the cells we are analysing.
#singlecell #bulkexperiment #singlecellworld
@singlecellworld
Getting knowledge on how the CytAssist from @10xGenomics works... #spatialtranscriptomics #cytassist
@singlecellworld
Companies focus on single-cell DNA:
- @MissionBio : droplet-based method, target approach, high-throughput
- @bioskryb : plate-based method, full genome, low-throughput
- @QIAGENscience: plate-based method, full genome, low-throughput
Any other?
#singlecell #scDNAseq
@singlecellworld
Why Is Sample Preparation Optimization So Important?
- Avoid cell damage or death
- Ensure⬆️cell suspension purity
- Avoid the lost of certain cell types
- ⬇️ experimental noise & bias
- Ensure ⬆️quality data
- ⬇️ the ⏱ and resources required for the #singlecell experiment
@singlecellworld
5 FACS hacks to maximize cell viability for #singlecell experiments 👇🏻
- 100um nozzle size
- collect cells in low binding tube with 50ul PBS-BSA
- 4°C #FACS tube collection holder
- PI to stain #deadcells
- Size-select cells (remove debris & doublets/multiplets)
#singlecellworld
@singlecellworld
Companies with commercial #scRNAseq kits
🟢 @10xGenomics
🟢 @Singleron_Bio
🟢 @BDBiosciences
🟢 @honeycombbio
🟢 @ParseBio
🟢 @Takara_Bio_EU
#singlecell #singlecelltranscriptomics #singlecellcompanies #scRNAkits
@singlecellworld
One of my hobbies...design and produce merchandise related with #science. Let me know if someday you need my help! #singlecell
@singlecellworld
Lovely #singlecell Valentine's postcards @ mostlyaboutscience.com 😉 @Moutinho_C @Sc_Ninjas @LGMartelotto
@singlecellworld
3 kits that can help ⬇️/remove dead cells from your #singlecell suspension👇
-Dead Cell Removal Kit @miltenyibiotec
-EasySep™ Dead Cell Removal (Annexin V) Kit @STEMCELLTech
-Dead Cell Removal Microbubble @Akadeum
Did you use other kit?
#samplepreparation #deadcells
@singlecellworld
🤓Terminology of the week 👇🏻
DOUBLET
- When 2 cells stick together and the technology treats them as a single cell, this is called a doublet.
#singlecell #singlecellsequencing #scRNAseq #scDNAseq #scATACseq #singlecelltrancriptomics #singlecellworld
@singlecellworld
I wish you all a happy 2023!! With a lot of successful single-cell experiments! 😉 #singlecell #singlecelltranscriptomics #singlecellrnasequencing #singlecelltechnology
@singlecellworld
Terminology of the week👉🏻BENCHMARKING
In #SingleCell sequencing is the process of assessing how good 1 method or workflow performs in comparison to other methods or processes used in this field. It allow us to identify the best methods to use on a specific project or application
@singlecellworld
DROPLET-based methods are the most commonly used in #singlecell research
🤔 BUT are the best? Not always.
Studying mRNA isoforms? 👉🏼PLATE-based methods
Studying neutrophils/ granulocytes? 👉🏼 (micro- nano- or pico- well based methods)